Spark® Multimode Microplate Reader - ready for any task

Watch the video to discover the power of Spark.

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“The best reader for analyzing drug candidates.”

"The Spark instrument has been the best reader for analyzing our samples, candidates for new drugs. Tecan support has been very helpful in optimizing the software applications to our needs."

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“A must have in the lab.”

"Easy to use and to achieve high quality, reproducible results."

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“It is handy to have in the lab.”

“The plate reader is easy to use and straight forward. Support usually comes pretty quickly too.”

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“Great for protein assays.”

"I have used the Tecan Spark plate reader for over a year. It is a very robust, easy-to-use instrument. When it comes to protein aggregation kinetics, life becomes so much easier with this instrument. I don’t have to worry about reproducibility, and we can monitor continuously, even at physiological temperature."

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“A multipurpose instrument for a wide array of assays including HTRF, AlphaScreen, and FP.”

"The instrument is easy to use. I especially love the z height functionality. It greatly improves sensitivity."

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“Easy to use and versatile!”

"It’s very easy to use. One can measure absorbance, luminescence, fluorescence and even RNA & DNA estimation in a single instrument. Spark really is awesome."

Features

The Spark multimode microplate reader offers solutions for virtually any life science research or drug discovery application.

Spark can be both freely configured and upgraded in the future, providing you an instrument that can meet your needs today and in the future.

Absorbance

With its High Speed Monochromator, Spark provides unparalleled wavelength accuracy for DNA and protein analysis. Spark offers full absorbance spectrum data – from 200 to 1,000 nm – in less than five seconds, excellent accuracy in the deep UV range (230-260 nm) for improved DNA/RNA analysis, and an OD range from 0-4 that ensures good linearity, requiring fewer dilutions and less manual pipetting.

Fluorescence

Spark offers industry leading sensitivity across the spectral range from its high performance PMT and combination of QuadX Monochromators with dichroic mirrors. Additionally, Spark‘s unique Fusion Optics allow flexible set-up of FP experiments using any combination of filter- or monochromator-based optics.

Luminescence

Spark offers standard or multi-color measurements using 40 user-selectable filters. A dedicated single photon counting PMT gives excellent sensitivity over a dynamic range of 10^9 without compromising other detection modes, while dedicated fibers for each plate format (96 to 1,536 wells) minimize crosstalk and give luminometer-like performance.

Automated Cell Imaging

Spark’s automated cell imaging and confluence calculations help you achieve more reliable results for your cell-based assays. It is possible to automatically image cells, measure confluence in a well, and inject a compound or measure GFP once a user-defined cell confluence is reached. Using Tecan’s Cell Chips™ Spark can automatically count a broad range of cells in suspension in less than 30 seconds.

Environmental Controls

Spark offers multiple environmental controls including an integrated Gas Control Module (GCM™), Humidity Cassettes, and an automated lid lifter that turn Spark into a reader/ incubator hybrid with flexible workflow automation capabilities.

Temperature Stability

Spark’s patented Te-Cool module allows you to perform assays at your specified temperature in the reader for long periods, even below ambient temperature. Precise cooling using Te-Cool empowers optimal performance of enzymatic assays, Alpha Technology assays, or kinetic assays using live organisms.

Benefits

The fully flexible detection platform with smart automation that’s made for your research today and tomorrow.

Flexibility

Spark allows for free combination of a broad range of modules, giving you the ability to configure the system to exactly match your needs.

Spark is upgradeable, ensuring an investment with the flexibility to grow to meet your future requirements.

Wide range of format flexibility with support for cuvettes, Cell Chips, NanoQuant Plate, and ANSI/SLAS microplates up to 1,536 wells.

Spark was designed with automation in mind. Enhanced features such as environmental controls, Spark-Stack™, and smart automation using SparkControl maximize walkaway time while giving you peace of mind.

Ease of Use

SparkControl’s touch-optimized, intuitive interface empowers fast, simple instrument operation at your fingertips.

SparkControl makes it easy to adjust parameters during a run, including environmental conditions such as temperature (even below ambient) and the CO2 and O2 levels inside the reader.

One-click applications streamline your workflows, getting you from sample to results faster than ever before.

Automated z-focus adjustment enhances the sensitivity of top-reading fluorescence intensity and fluorescence polarization modes, significantly improving the quality of results.

Innovation

NanoQuant Plate™ - the only low volume plate on the market that is 100% calibration free. NanoQuant allows parallel quantification and analysis of up to 16 nucleic acid or protein samples, in volumes as little as 2 µl.

Fusion Optics allow any combination of filters or monochromators on both the excitation AND emission sides for every fluorescence measurement; you no longer have to choose between sensitivity OR flexibility for your assays.

Patented integrated Gas Control Module (GCM™) allows automated control of CO2 and O2 partial pressures inside the reader chamber.

The big picture for your cell-based assays: Fluorescence imaging and Spark

Ready for Live cell assays: The combination of the Spark multimode reader with fluorescence imaging capabilities offers the first live cell plate reader for cytometry, Spark Cyto.

The Spark Cyto combines three main elements:

  • A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats.
  • Real-time experimental control (REC) that automatically activates a procedure as soon as a specific data value is reached.
  • The flexibility of a high end multimode plate reader with environmental control.
      

Key applications include:

  • Nuclei counting
  • GFP transfection efficiency
  • Cell viability and cell death
  • Apoptosis
  • Cell roughness

Spark Multimode Reader Specifications

Systems overview of Spark Multimode Plate Readers. Please see typical performance values in the download below for more details. Spark is the fully configurable platform that gives you the freedom to personalize your own reader.

Features

Details

Assays

Absorbance
High Speed Monochromators ELISA, DNA, protein quantification
Fluorescence
Filter based
QuadX Monochromator based
Fusion Optics (filter + monochromator)
• Fluorescence Intensity
• Fluorescence Polarization
• Time-Resolved Fluorescence
• HTRF®, LanthaScreen®
• 3D spectral scans
Luminescence
Dedicated PMT - single photon counting
Multi-color monochromator (40 filters)
Glow , flash, multicolor assays, and spectral scans; BRET™, DLR™
AlphaScreen®
High performance laser excitation
IR sensor based temperature correction
AlphaScreen®, AlphaLisa®, AlphaPLex®
Imaging
Brightfield imaging
4x objective
Cell imaging
Confluence assessment
Cell Counting
Labware
1-1536 well plates, cuvettes
Tecan Nanoquant plate (2µl volume)
Tecan CellChips
Environmental Control
Temperature Control RT +3°C -42°CAmbient +42°C
Te-Cool active regulation form 18-42°C)
CO2/O2 control
 
Liquid Dispenser
2 syringes
Reagent heater and stirrer
TYPICAL PERFORMANCE VALUES


Free Download:
Demystifying the Black Box – The Microplate Reader in the Spotlight

Microplate readers are a workhorse in your lab, but they tend to get overlooked as just a “black box”. The sophisticated components and precisely engineered mechanisms required to produce your data sets definitely deserve more attention.

Take your understanding one step further to unlock your reader’s max potential and discover new ways to optimize your assays by downloading our poster below.
  

DOWNLOAD POSTER

 

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A Tecan representative will contact you to help you work out which Spark configurations are right for you and your laboratory.
We are excited to hear from you.